Paddington y la familia Brown viajan a Perú para encontrar a la tía Lucy, cuya misteriosa desaparición puede estar relacionada con una legendaria ciudad perdida.

Paddington, now happily settled with the Brown family and a popular member of the local community, picks up a series of odd jobs to buy the perfect present for his Aunt Lucy's 100th birthday, only for the gift to be stolen.

A young Peruvian bear travels to London in search of a home. Finding himself lost and alone at Paddington Station, he meets the kindly Brown family, who offer him a temporary haven.